Journal: Cell reports

Article Title: Noncanonical genomic imprinting in the monoamine system determines naturalistic foraging and brain-adrenal axis functions

doi: 10.1016/j.celrep.2022.110500

Figure Lengend Snippet: (A) The images show mixed DDC+ cell populations with paternal allele (blue arrows), maternal allele (pink arrows), and biallelic expression (yellow arrows) in the adrenal medulla. Low-magnification images of Ddc EGFP/V5 and Ddc V5/EGFP cells show groups of biallelic cells and many paternal-allele-expressing cells (size bar is 100 μm). Insets show high-magnification images of cells with different allelic expression effects. Size bar is 20 μm. (B–E) The schematic (B) shows the experimental design for RNA-seq profiling to uncover molecular pathways differentially controlled by maternal versus paternal Ddc alleles in the female and male adrenal gland (n = 4). The heatmap (C) shows the RNA-seq differential expression results for genes with a significant interaction effect between genotype and parental crosses (generalized linear model, DESeq2, false discovery rate [FDR] < 5%). The barplot (D) shows the results of a post-test revealing the number of genes with significant differential expression from comparisons of maternal (red) or paternal (blue) Ddc allelic mutants with +/+ littermates (t test post-test, DESeq2, FDR < 5%). The Venn diagrams in (E) compare the significant GO terms found for differentially expressed genes in Ddc maternal (red) versus paternal (blue) allelic mutant females and males. (F and G) The barplots show the top Gene Ontology biological process enrichments for the female (F) and male (G) differentially expressed gene sets in (D) (TOPGO elimKS test, p < 0.01). See . (H and I) The barplots show ELISA-detected levels of DA, NE, and E in the urine of female (H) and male (I) mice with null maternal (red) or paternal (blue) Ddc alleles compared with +/+ littermates (creatinine-normalized values shown, t test, n = 20). *p < 0.05, **p < 0.01.

Article Snippet: Catecholamine ELISA kit , Eagle Biosciences , KA1880.

Techniques: Expressing, RNA Sequencing, Quantitative Proteomics, Mutagenesis, Enzyme-linked Immunosorbent Assay

Journal: Cell reports

Article Title: Noncanonical genomic imprinting in the monoamine system determines naturalistic foraging and brain-adrenal axis functions

doi: 10.1016/j.celrep.2022.110500

Figure Lengend Snippet:

Article Snippet: Catecholamine ELISA kit , Eagle Biosciences , KA1880.

Techniques: Virus, Recombinant, Catecholamine ELISA, Construct, CRISPR, Plasmid Preparation, Software

Journal: Scientific Reports

Article Title: Adenosine deaminase modulates metabolic remodeling and orchestrates joint destruction in rheumatoid arthritis

doi: 10.1038/s41598-021-94607-5

Figure Lengend Snippet: Showing ADA Activity and Cytokine levels in Rheumatoid arthritis patients. ( a ) ADA activity in RA patients representing group with Low ADA and High ADA when compared to healthy controls. ( b ) ADA activity of two isoenzymes ADA 1 and ADA 2 (*representing a p value of ≤ 0.05) ( c ) Levels of Cytokines in the serum of RA patients. ( d ) One-way ANOVA analysis of Cytokines and ADA levels in healthy controls, Low ADA and High ADA RA patients. (p-value of ≤ 0.05 is considered significant). All the plots were made using Origin 2020.

Article Snippet: TNFα (Cat. No. CHC1753), TGFβ (Cat. No. CHC1683), IFNγ (Cat. No. CHC1233), and IL-10 (Cat. No. CHC1323) (Invitrogen Cytoset Antibody Pair Kit , California, USA) and IL-6 (Cat. No. 900 M16) and sRANKL (Cat. No. 900 M142) (Peprotech Mini ABTS ELISA Kit, New Jersey, USA) were estimated using manufacturers’ protocols and calculated by the 4-parameter curve-fit method.

Techniques: Activity Assay

Journal: Scientific Reports

Article Title: Adenosine deaminase modulates metabolic remodeling and orchestrates joint destruction in rheumatoid arthritis

doi: 10.1038/s41598-021-94607-5

Figure Lengend Snippet: ADA is involved in joint remodeling in rheumatoid arthritis—in vitro studies to investigate the role of ADA in joint remodeling in the affected joint. ( a ) Increased proliferation of Synoviocytes, ( b ) Decreased cell viability in primary chondrocytes. ( c ) Increased Osteoclast differentiation of macrophages and ( d ) qPCR analysis showing the expression of osteoclast markers after 72 h exposure to ADA. ( e ) Increased cytokine levels in the spent medium after 72 exposure to ADA. ( f ) P2Y 2 , P2Y 4 , and P2Y 6 receptor expression in macrophages after 72 h of ADA exposure. (showing *representing a p value of ≤ 0.05, and **representing a p value of ≤ 0.005). ( g ) Increased ROS in macrophages after ADA exposure (p values of ADA to CTRL on all time points are ≤ 0.005). All the plots were made using Origin 2020.

Article Snippet: TNFα (Cat. No. CHC1753), TGFβ (Cat. No. CHC1683), IFNγ (Cat. No. CHC1233), and IL-10 (Cat. No. CHC1323) (Invitrogen Cytoset Antibody Pair Kit , California, USA) and IL-6 (Cat. No. 900 M16) and sRANKL (Cat. No. 900 M142) (Peprotech Mini ABTS ELISA Kit, New Jersey, USA) were estimated using manufacturers’ protocols and calculated by the 4-parameter curve-fit method.

Techniques: In Vitro, Expressing

Journal: Pakistan Journal of Medical Sciences

Article Title: Importance of scattered white spots detected in the duodenum and their relationship with zonulin

doi: 10.12669/pjms.40.11.9800

Figure Lengend Snippet: Demographic characteristics and laboratory results of the patients.

Article Snippet: Zonulin levels in the serum samples were measured using commercial BT-LAB Human Zonulin ELISA kits (Cat.No: E1117Hu) (Shanghai Crystal Day Biotech Co., Ltd, Shanghai, China).

Techniques:

Journal: Pakistan Journal of Medical Sciences

Article Title: Importance of scattered white spots detected in the duodenum and their relationship with zonulin

doi: 10.12669/pjms.40.11.9800

Figure Lengend Snippet: Zonulin value.

Article Snippet: Zonulin levels in the serum samples were measured using commercial BT-LAB Human Zonulin ELISA kits (Cat.No: E1117Hu) (Shanghai Crystal Day Biotech Co., Ltd, Shanghai, China).

Techniques:

Journal: Pakistan Journal of Medical Sciences

Article Title: Importance of scattered white spots detected in the duodenum and their relationship with zonulin

doi: 10.12669/pjms.40.11.9800

Figure Lengend Snippet: Comparison of gastric and duodenal biopsy results with serum zonulin levels.

Article Snippet: Zonulin levels in the serum samples were measured using commercial BT-LAB Human Zonulin ELISA kits (Cat.No: E1117Hu) (Shanghai Crystal Day Biotech Co., Ltd, Shanghai, China).

Techniques: Comparison